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expression vector pgs21a  (GenScript corporation)

 
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    GenScript corporation expression vector pgs21a
    Expression Vector Pgs21a, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+pgs21a/pgs+21a/pmc03225368-39-6-9
    Average 90 stars, based on 1 article reviews
    expression vector pgs21a - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli
    Article Snippet: E. coli BL21, BL21(DE3) and Rosetta 2(DE3) competent cells were obtained from Novagen (Madison, WI). .. Expression vector pGS21a and mouse anti His-tag monoclonal antibody (mAb) was from GenScript Corporation (Piscataway, NJ). .. Restriction enzymes EcoR I and Xho I were from Boehringer-Mannheim (GmbH, Germany).

    Article Title: A Conformational Switch in the Active Site of BT_2972, a Methyltransferase from an Antibiotic Resistant Pathogen B. thetaiotaomicron
    Article Snippet: .. The BT_2972 gene was cloned into expression vector pGS21a (GeneScript, USA) and the recombinant plasmid was transformed into E. coli BL21 (DE3) competent cells and plated onto ampicillin-containing agar plates . ..

    Plasmid Preparation:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli
    Article Snippet: E. coli BL21, BL21(DE3) and Rosetta 2(DE3) competent cells were obtained from Novagen (Madison, WI). .. Expression vector pGS21a and mouse anti His-tag monoclonal antibody (mAb) was from GenScript Corporation (Piscataway, NJ). .. Restriction enzymes EcoR I and Xho I were from Boehringer-Mannheim (GmbH, Germany).

    Article Title: A Conformational Switch in the Active Site of BT_2972, a Methyltransferase from an Antibiotic Resistant Pathogen B. thetaiotaomicron
    Article Snippet: .. The BT_2972 gene was cloned into expression vector pGS21a (GeneScript, USA) and the recombinant plasmid was transformed into E. coli BL21 (DE3) competent cells and plated onto ampicillin-containing agar plates . ..

    Clone Assay:

    Article Title: A Conformational Switch in the Active Site of BT_2972, a Methyltransferase from an Antibiotic Resistant Pathogen B. thetaiotaomicron
    Article Snippet: .. The BT_2972 gene was cloned into expression vector pGS21a (GeneScript, USA) and the recombinant plasmid was transformed into E. coli BL21 (DE3) competent cells and plated onto ampicillin-containing agar plates . ..

    Recombinant:

    Article Title: A Conformational Switch in the Active Site of BT_2972, a Methyltransferase from an Antibiotic Resistant Pathogen B. thetaiotaomicron
    Article Snippet: .. The BT_2972 gene was cloned into expression vector pGS21a (GeneScript, USA) and the recombinant plasmid was transformed into E. coli BL21 (DE3) competent cells and plated onto ampicillin-containing agar plates . ..

    Transformation Assay:

    Article Title: A Conformational Switch in the Active Site of BT_2972, a Methyltransferase from an Antibiotic Resistant Pathogen B. thetaiotaomicron
    Article Snippet: .. The BT_2972 gene was cloned into expression vector pGS21a (GeneScript, USA) and the recombinant plasmid was transformed into E. coli BL21 (DE3) competent cells and plated onto ampicillin-containing agar plates . ..



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    expression vector pgs21a  (GenScript corporation)
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    GenScript corporation expression vector pgs21a
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    https://www.bioz.com/product/expression+vector+pgs21a/pgs+21a/pmc03225368-39-6-9
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    bacterial expression vector pgs21a  (GenScript corporation)
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    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
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    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
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    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

    A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, Recombinant, Plasmid Preparation

    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

    A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, Recombinant, Plasmid Preparation